Assessment of Collagen III as a Non-Invasive Biomarker in HCV Induced Liver Fibrotic Patients

Abstract Introduction/Objective Liver biopsy is still the gold typical for assessing hepatic fibrosis. Non-invasive serum parameters measurement leads to essential changes in diagnosis. This procedure painful, carries a risk of bleeding and infection, accompanied by high sampling error rate. New biomarkers that can accurately predict stage fibrosis are urgently needed. Objectives study aimed identify, characterize, detect collagen III patients with HCV-induced liver healthy subjects. Methods/Case Report The subjects were divided into two groups; group1: included 118 (60 males 58 females). Group2: 50 comparable control (27 23 Collagen was identified samples group1 at 70 kDa using polyclonal antibody western blot analysis techniques. Additionally, it purified from electroelution technique preparative slab gels. antigen gives single sharp band when resolved SDS–PAGE (sodium dodecyl sulfate-polyacrylamide gel electrophoresis) stained coomassie blue. showed reactivity towards antibody. We used ELISA method quantify III, dose-response curve as function concentration done. Results (if Case Study enter NA) 62% patients, 38 % negative (false negative) group one. For two, 96% total volunteers 4% positive positive). mean ± SD (8.38±3.56) (3.2±0.59) groups 1 2, respectively, (P<0.0001). Conclusion non-invasive assessment has shown promising diagnostic prognostic potential.